Reproductive Biology and Endocrinology volume 22 , Article number: Cite this article. Metrics details. Restoring the function of the ovary is important for chemotherapy-induced ovarian failure COF patients. Stem cell and extracellular vesicles EVs therapy show promise but need further improvement.
Human umbilical cord mesenchymal stem cells hUC-MSCs were primarily cultured and further three-dimensional 3D cultured using an ultra-low attachment surface method. The expression levels of nutritional cytokines and immunomodulatory and stemness-related genes of 3D-cultured hUC-MSCs were analyzed.
EVs were isolated by ultracentrifugation and characterized. Ovaries were decellularized with sodium dodecyl sulfate to obtain extracellular matrix ECM. RNA sequencing was used to analyze the expression changes of genes before and after treatment.
After culturing in ultra-low attachment dishes, hUC-MSCs aggregated into spheroids and significantly upregulated the expression levels of immunomodulatory and stemness-related genes. The total EVs yield was also upregulated 5. In recent years, the burden of cancer has risen worldwide in adults, adolescents, and children [ 1 , 2 ].
Current cancer treatments include chemotherapy, radiotherapy, surgery, and immunotherapy [ 3 ]. Chemotherapy is a common treatment option [ 4 ].
